FISH testing (fluorescence in situ hybridization) is a way of testing myeloma genetics in individual patients. It uses special fluorescent dyes that only attach to specific parts of chromosomes.
What does positive FISH test mean?
FISH testing usually returns one of two results: positive or negative. Positive means your breast cancer cells make too much HER2 and your doctor should treat you with drugs that target that protein. Negative means the protein isn’t involved in the growth of your tumor.
What does an abnormal fish test mean?
FISH also can identify chromosomal abnormalities in leukemias, including chronic lymphocytic leukemia (CLL) cells, some of which are associated with aggressive forms of the disease. Patients with more aggressive forms of CLL may need urgent treatment, while those with less aggressive forms may only require observation.
How do you read FISH results?
How your doctor interprets this test is as follows:
- A result of 0 is negative.
- A result of 1+ is also negative.
- A result of 2+ is considered equivocal (uncertain).
- A result of 3+ is positive.
What is FISH test in bone marrow biopsy?
Fluorescent in situ hybridization (FISH) is a technique which complements conventional cytogenetic banding analysis by allowing the evaluation of cells in interphase as well as metaphase. This technique has been used to study air-dried peripheral blood and bone marrow aspirate smears.
Is the FISH test accurate?
Accuracy and limitations. Prenatal interphase FISH testing is highly accurate, with reported false-positive and -negative rates usually less than 1%. The main problem, however, is that not all specimens are informative. Uninformative rates will vary among laboratories, but rates of 3% to 10% are considered typical.
What does FISH mean in medical terms?
En Español. Fluorescence in situ hybridization (FISH) provides researchers with a way to visualize and map the genetic material in an individual’s cells, including specific genes or portions of genes. This may be used for understanding a variety of chromosomal abnormalities and other genetic mutations.
What is a FISH urine test?
The innovative FISH test – which stands for fluorescence in situ hybridization (FISH) — utilizes a urine sample and colored dyes that allow for detection of cancer-promoting abnormalities under a fluorescent microscope in the laboratory.
Is a FISH test a blood test?
A blood sample is used for a FISH test for CLL. Tests that look at changes in genes or chromosomes are called cytogenetic tests. The most important change is when a gene called TP53 is missing or does not work.
How do I read myeloma panels?
High levels mean the disease is more advanced and may indicate a poor prognosis. The presence of any M spike is abnormal and shows the presence of an abnormal clone of plasma cells. If the amount of protein is ≥30 g/L and/or there are other disease symptoms, the patient may have myeloma.
What does HER2 equivocal 2+ mean?
If the IHC result is 2+, the HER2 status of the tumor is not clear and is called “equivocal.” This means that the HER2 status needs to be tested with FISH to clarify the result.
How does FISH assay work?
Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.
What is a FISH score?
Fluorescence in situ hybridization (FISH) is a test that “maps” the genetic material in a person’s cells. This test can be used to visualize specific genes or portions of genes. FISH testing is done on breast cancer tissue removed during biopsy to see if the cells have extra copies of the HER2 gene.
How do you read HER2 FISH results?
- Positive HER2 amplification: FISH ratio is greater than 2.2 or HER2 gene copy is greater than 6.0.
- Equivocal HER2 amplification: FISH ratio of 1.8-2.2 or HER2 gene copy of 4.0-6.0.
- Negative HER2 amplification: FISH ratio is less than 1.8 or HER2 gene copy of less than 4.0.
What is FISH used to diagnose?
FISH is applied to detect genetic abnormalities that include different characteristic gene fusions or the presence of an abnormal number of chromosomes in a cell or loss of a chromosomal region or a whole chromosome.